The Bee was seen in the episode "Evicted!," when Jake, along with Finn, tried to move into its hive by forcing his foot into the entrance (during House Hunting Song). The bee vomits honey on both of them to scare them away. Later, at Finn and Jake's cave party, the bee can be seen flying into Mr. Cupcake's frosting and getting stuck in it.
The Bee looks similar to any regular bee, complete with black and yellow stripes, a pair of wings, three pairs of legs, and a stinger, but it has no antennae.
- A similar-looking bee can be seen in the title card for "Death in Bloom" and in the Adventure Time Ending Theme.
- Another similar-looking bee can be found in Pendleton Wards other animated series of shorts, Bravest Warriors, as Chris' weapon animal.
- It is used as temporary pet in Adventure Time: Hey Ice King! Why'd you steal our garbage?!!